anti grik3 Search Results


anti grik3  (Alomone Labs)
93
Alomone Labs anti grik3
iGluR activity, but not LRP6 overexpression, control cell polarization to Wnt3a beads, and pESC–TSC approximation. (A and B) Representative images of pESCs stained with antibodies against LRP6 (A) and β-catenin (B), and with phalloidin (F-actin). Inserts are magnification of boxes, contrast enhanced for clarity. Scale bars, 20 µm for larger images, 5 µm for inserts. (C) The percentage of cytonemes positive for LRP6 or β-catenin in pESCs. n = 62 cells. (D) Lrp6 RNA expression levels in control (CNTRL) pESCs or pESCs transiently overexpressing LRP6-eGFP (LRP6 OE), presented as fold-change to CNTRL pESCs. Bars are mean of n = 3 experiments. Error bars are SEM. Statistical significance calculated by unpaired two-sided t test: ***, P < 0.001. (E) Representative images of CNTRL or LRP6-eGFP overexpressing pESCs, stained with antibodies against LRP6. BF is brightfield. Yellow arrowhead indicates high levels of LRP6 in the cytoneme. Images are maximum-intensity projections presented at equal intensity range to allow comparison between panels. Scale bars, 20 µm. (F) The percentage of reactive interactions (defined in ) between ESCs, CNTRL pESCs, pESCs overexpressing LRP6 (LRP6 OE), or pESCs treated with 100 µM kainate and TSCs. n ≥ 58 cells pooled from ≥3 independent experiments. Statistical significance calculated by multiple Fisher’s exact two-sided tests: ****, P < 0.0001. (G) Representative images of ESCs contacting a Wnt3a bead at the base of the cytonemes, stained with antibodies against LRP6 or β-catenin (cyan) and GluA3, GluA4, GluK1, and <t>GluK3.</t> Bead is black sphere in brightfield (BF) panel and is highlighted with a dashed yellow circle. Scale bars, 10 µm. (H and I) The percentage of control ESCs (CTRL, blue), 10 µM CNQX-treated ESCs (green), pESCs (CTRL, orange), or pESCs treated with 100 µM kainate (pink) with polarized presentation of LRP6 (H) or β-catenin (I) upon Wnt3a bead contact. n ≥ 41 cells. Further quantification is shown in . Statistical significance calculated by multiple Fisher’s exact two-sided tests: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. (J) The percentage of ESCs (blue) or pESCs (orange) with polarized distribution of both Wnt/β-catenin pathway components and GluA3, GluA4, GluK1, or GluK3. n ≥ 26 cells. Statistical significance calculated by multiple Fisher’s exact two-sided tests: **, P < 0.01. (K and L) Representative frames of a time course live-cell imaging experiment showing a pESC (magenta) treated with 100 µM kainate contacting a TSC (green) through a cytoneme, approaching it, and then separating (K). Time is minutes, and yellow dashed line indicates distance between cells. Scare bar, 20 µm. Plot on L indicates pESC-TSC distance over time (cell in K only). Arrows point to distance at initial contact (X c ) and distance at 50 min after initial cytoneme-mediated contact (X c+50 ). (M) The difference in distance between CNTRL pESCs (orange) or 100 µM kainate–treated pESCs (KA, pink) and TSCs at the initial cytoneme-mediated contact (X c ) or 50 min after contact (X c+50 , Δ Distance = X c+50 – X c ). Bars are mean of n ≥ 58 cells pooled from ≥3 experiments. Error bars are SEM. Asterisks indicate statistical significance calculated by unpaired two-sided t test: **, P < 0.01.
Anti Grik3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti grik3/product/Alomone Labs
Average 93 stars, based on 1 article reviews
anti grik3 - by Bioz Stars, 2026-02
93/100 stars
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anti grik3  (Cusabio)
90
Cusabio anti grik3
iGluR activity, but not LRP6 overexpression, control cell polarization to Wnt3a beads, and pESC–TSC approximation. (A and B) Representative images of pESCs stained with antibodies against LRP6 (A) and β-catenin (B), and with phalloidin (F-actin). Inserts are magnification of boxes, contrast enhanced for clarity. Scale bars, 20 µm for larger images, 5 µm for inserts. (C) The percentage of cytonemes positive for LRP6 or β-catenin in pESCs. n = 62 cells. (D) Lrp6 RNA expression levels in control (CNTRL) pESCs or pESCs transiently overexpressing LRP6-eGFP (LRP6 OE), presented as fold-change to CNTRL pESCs. Bars are mean of n = 3 experiments. Error bars are SEM. Statistical significance calculated by unpaired two-sided t test: ***, P < 0.001. (E) Representative images of CNTRL or LRP6-eGFP overexpressing pESCs, stained with antibodies against LRP6. BF is brightfield. Yellow arrowhead indicates high levels of LRP6 in the cytoneme. Images are maximum-intensity projections presented at equal intensity range to allow comparison between panels. Scale bars, 20 µm. (F) The percentage of reactive interactions (defined in ) between ESCs, CNTRL pESCs, pESCs overexpressing LRP6 (LRP6 OE), or pESCs treated with 100 µM kainate and TSCs. n ≥ 58 cells pooled from ≥3 independent experiments. Statistical significance calculated by multiple Fisher’s exact two-sided tests: ****, P < 0.0001. (G) Representative images of ESCs contacting a Wnt3a bead at the base of the cytonemes, stained with antibodies against LRP6 or β-catenin (cyan) and GluA3, GluA4, GluK1, and <t>GluK3.</t> Bead is black sphere in brightfield (BF) panel and is highlighted with a dashed yellow circle. Scale bars, 10 µm. (H and I) The percentage of control ESCs (CTRL, blue), 10 µM CNQX-treated ESCs (green), pESCs (CTRL, orange), or pESCs treated with 100 µM kainate (pink) with polarized presentation of LRP6 (H) or β-catenin (I) upon Wnt3a bead contact. n ≥ 41 cells. Further quantification is shown in . Statistical significance calculated by multiple Fisher’s exact two-sided tests: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. (J) The percentage of ESCs (blue) or pESCs (orange) with polarized distribution of both Wnt/β-catenin pathway components and GluA3, GluA4, GluK1, or GluK3. n ≥ 26 cells. Statistical significance calculated by multiple Fisher’s exact two-sided tests: **, P < 0.01. (K and L) Representative frames of a time course live-cell imaging experiment showing a pESC (magenta) treated with 100 µM kainate contacting a TSC (green) through a cytoneme, approaching it, and then separating (K). Time is minutes, and yellow dashed line indicates distance between cells. Scare bar, 20 µm. Plot on L indicates pESC-TSC distance over time (cell in K only). Arrows point to distance at initial contact (X c ) and distance at 50 min after initial cytoneme-mediated contact (X c+50 ). (M) The difference in distance between CNTRL pESCs (orange) or 100 µM kainate–treated pESCs (KA, pink) and TSCs at the initial cytoneme-mediated contact (X c ) or 50 min after contact (X c+50 , Δ Distance = X c+50 – X c ). Bars are mean of n ≥ 58 cells pooled from ≥3 experiments. Error bars are SEM. Asterisks indicate statistical significance calculated by unpaired two-sided t test: **, P < 0.01.
Anti Grik3, supplied by Cusabio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti grik3/product/Cusabio
Average 90 stars, based on 1 article reviews
anti grik3 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
rabbit anti-grik3 a10701  (ABclonal Biotechnology)
90
ABclonal Biotechnology rabbit anti-grik3 a10701
iGluR activity, but not LRP6 overexpression, control cell polarization to Wnt3a beads, and pESC–TSC approximation. (A and B) Representative images of pESCs stained with antibodies against LRP6 (A) and β-catenin (B), and with phalloidin (F-actin). Inserts are magnification of boxes, contrast enhanced for clarity. Scale bars, 20 µm for larger images, 5 µm for inserts. (C) The percentage of cytonemes positive for LRP6 or β-catenin in pESCs. n = 62 cells. (D) Lrp6 RNA expression levels in control (CNTRL) pESCs or pESCs transiently overexpressing LRP6-eGFP (LRP6 OE), presented as fold-change to CNTRL pESCs. Bars are mean of n = 3 experiments. Error bars are SEM. Statistical significance calculated by unpaired two-sided t test: ***, P < 0.001. (E) Representative images of CNTRL or LRP6-eGFP overexpressing pESCs, stained with antibodies against LRP6. BF is brightfield. Yellow arrowhead indicates high levels of LRP6 in the cytoneme. Images are maximum-intensity projections presented at equal intensity range to allow comparison between panels. Scale bars, 20 µm. (F) The percentage of reactive interactions (defined in ) between ESCs, CNTRL pESCs, pESCs overexpressing LRP6 (LRP6 OE), or pESCs treated with 100 µM kainate and TSCs. n ≥ 58 cells pooled from ≥3 independent experiments. Statistical significance calculated by multiple Fisher’s exact two-sided tests: ****, P < 0.0001. (G) Representative images of ESCs contacting a Wnt3a bead at the base of the cytonemes, stained with antibodies against LRP6 or β-catenin (cyan) and GluA3, GluA4, GluK1, and <t>GluK3.</t> Bead is black sphere in brightfield (BF) panel and is highlighted with a dashed yellow circle. Scale bars, 10 µm. (H and I) The percentage of control ESCs (CTRL, blue), 10 µM CNQX-treated ESCs (green), pESCs (CTRL, orange), or pESCs treated with 100 µM kainate (pink) with polarized presentation of LRP6 (H) or β-catenin (I) upon Wnt3a bead contact. n ≥ 41 cells. Further quantification is shown in . Statistical significance calculated by multiple Fisher’s exact two-sided tests: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. (J) The percentage of ESCs (blue) or pESCs (orange) with polarized distribution of both Wnt/β-catenin pathway components and GluA3, GluA4, GluK1, or GluK3. n ≥ 26 cells. Statistical significance calculated by multiple Fisher’s exact two-sided tests: **, P < 0.01. (K and L) Representative frames of a time course live-cell imaging experiment showing a pESC (magenta) treated with 100 µM kainate contacting a TSC (green) through a cytoneme, approaching it, and then separating (K). Time is minutes, and yellow dashed line indicates distance between cells. Scare bar, 20 µm. Plot on L indicates pESC-TSC distance over time (cell in K only). Arrows point to distance at initial contact (X c ) and distance at 50 min after initial cytoneme-mediated contact (X c+50 ). (M) The difference in distance between CNTRL pESCs (orange) or 100 µM kainate–treated pESCs (KA, pink) and TSCs at the initial cytoneme-mediated contact (X c ) or 50 min after contact (X c+50 , Δ Distance = X c+50 – X c ). Bars are mean of n ≥ 58 cells pooled from ≥3 experiments. Error bars are SEM. Asterisks indicate statistical significance calculated by unpaired two-sided t test: **, P < 0.01.
Rabbit Anti Grik3 A10701, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-grik3 a10701/product/ABclonal Biotechnology
Average 90 stars, based on 1 article reviews
rabbit anti-grik3 a10701 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


iGluR activity, but not LRP6 overexpression, control cell polarization to Wnt3a beads, and pESC–TSC approximation. (A and B) Representative images of pESCs stained with antibodies against LRP6 (A) and β-catenin (B), and with phalloidin (F-actin). Inserts are magnification of boxes, contrast enhanced for clarity. Scale bars, 20 µm for larger images, 5 µm for inserts. (C) The percentage of cytonemes positive for LRP6 or β-catenin in pESCs. n = 62 cells. (D) Lrp6 RNA expression levels in control (CNTRL) pESCs or pESCs transiently overexpressing LRP6-eGFP (LRP6 OE), presented as fold-change to CNTRL pESCs. Bars are mean of n = 3 experiments. Error bars are SEM. Statistical significance calculated by unpaired two-sided t test: ***, P < 0.001. (E) Representative images of CNTRL or LRP6-eGFP overexpressing pESCs, stained with antibodies against LRP6. BF is brightfield. Yellow arrowhead indicates high levels of LRP6 in the cytoneme. Images are maximum-intensity projections presented at equal intensity range to allow comparison between panels. Scale bars, 20 µm. (F) The percentage of reactive interactions (defined in ) between ESCs, CNTRL pESCs, pESCs overexpressing LRP6 (LRP6 OE), or pESCs treated with 100 µM kainate and TSCs. n ≥ 58 cells pooled from ≥3 independent experiments. Statistical significance calculated by multiple Fisher’s exact two-sided tests: ****, P < 0.0001. (G) Representative images of ESCs contacting a Wnt3a bead at the base of the cytonemes, stained with antibodies against LRP6 or β-catenin (cyan) and GluA3, GluA4, GluK1, and GluK3. Bead is black sphere in brightfield (BF) panel and is highlighted with a dashed yellow circle. Scale bars, 10 µm. (H and I) The percentage of control ESCs (CTRL, blue), 10 µM CNQX-treated ESCs (green), pESCs (CTRL, orange), or pESCs treated with 100 µM kainate (pink) with polarized presentation of LRP6 (H) or β-catenin (I) upon Wnt3a bead contact. n ≥ 41 cells. Further quantification is shown in . Statistical significance calculated by multiple Fisher’s exact two-sided tests: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. (J) The percentage of ESCs (blue) or pESCs (orange) with polarized distribution of both Wnt/β-catenin pathway components and GluA3, GluA4, GluK1, or GluK3. n ≥ 26 cells. Statistical significance calculated by multiple Fisher’s exact two-sided tests: **, P < 0.01. (K and L) Representative frames of a time course live-cell imaging experiment showing a pESC (magenta) treated with 100 µM kainate contacting a TSC (green) through a cytoneme, approaching it, and then separating (K). Time is minutes, and yellow dashed line indicates distance between cells. Scare bar, 20 µm. Plot on L indicates pESC-TSC distance over time (cell in K only). Arrows point to distance at initial contact (X c ) and distance at 50 min after initial cytoneme-mediated contact (X c+50 ). (M) The difference in distance between CNTRL pESCs (orange) or 100 µM kainate–treated pESCs (KA, pink) and TSCs at the initial cytoneme-mediated contact (X c ) or 50 min after contact (X c+50 , Δ Distance = X c+50 – X c ). Bars are mean of n ≥ 58 cells pooled from ≥3 experiments. Error bars are SEM. Asterisks indicate statistical significance calculated by unpaired two-sided t test: **, P < 0.01.

Journal: The Journal of Cell Biology

Article Title: Pluripotency state regulates cytoneme selectivity and self-organization of embryonic stem cells

doi: 10.1083/jcb.202005095

Figure Lengend Snippet: iGluR activity, but not LRP6 overexpression, control cell polarization to Wnt3a beads, and pESC–TSC approximation. (A and B) Representative images of pESCs stained with antibodies against LRP6 (A) and β-catenin (B), and with phalloidin (F-actin). Inserts are magnification of boxes, contrast enhanced for clarity. Scale bars, 20 µm for larger images, 5 µm for inserts. (C) The percentage of cytonemes positive for LRP6 or β-catenin in pESCs. n = 62 cells. (D) Lrp6 RNA expression levels in control (CNTRL) pESCs or pESCs transiently overexpressing LRP6-eGFP (LRP6 OE), presented as fold-change to CNTRL pESCs. Bars are mean of n = 3 experiments. Error bars are SEM. Statistical significance calculated by unpaired two-sided t test: ***, P < 0.001. (E) Representative images of CNTRL or LRP6-eGFP overexpressing pESCs, stained with antibodies against LRP6. BF is brightfield. Yellow arrowhead indicates high levels of LRP6 in the cytoneme. Images are maximum-intensity projections presented at equal intensity range to allow comparison between panels. Scale bars, 20 µm. (F) The percentage of reactive interactions (defined in ) between ESCs, CNTRL pESCs, pESCs overexpressing LRP6 (LRP6 OE), or pESCs treated with 100 µM kainate and TSCs. n ≥ 58 cells pooled from ≥3 independent experiments. Statistical significance calculated by multiple Fisher’s exact two-sided tests: ****, P < 0.0001. (G) Representative images of ESCs contacting a Wnt3a bead at the base of the cytonemes, stained with antibodies against LRP6 or β-catenin (cyan) and GluA3, GluA4, GluK1, and GluK3. Bead is black sphere in brightfield (BF) panel and is highlighted with a dashed yellow circle. Scale bars, 10 µm. (H and I) The percentage of control ESCs (CTRL, blue), 10 µM CNQX-treated ESCs (green), pESCs (CTRL, orange), or pESCs treated with 100 µM kainate (pink) with polarized presentation of LRP6 (H) or β-catenin (I) upon Wnt3a bead contact. n ≥ 41 cells. Further quantification is shown in . Statistical significance calculated by multiple Fisher’s exact two-sided tests: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. (J) The percentage of ESCs (blue) or pESCs (orange) with polarized distribution of both Wnt/β-catenin pathway components and GluA3, GluA4, GluK1, or GluK3. n ≥ 26 cells. Statistical significance calculated by multiple Fisher’s exact two-sided tests: **, P < 0.01. (K and L) Representative frames of a time course live-cell imaging experiment showing a pESC (magenta) treated with 100 µM kainate contacting a TSC (green) through a cytoneme, approaching it, and then separating (K). Time is minutes, and yellow dashed line indicates distance between cells. Scare bar, 20 µm. Plot on L indicates pESC-TSC distance over time (cell in K only). Arrows point to distance at initial contact (X c ) and distance at 50 min after initial cytoneme-mediated contact (X c+50 ). (M) The difference in distance between CNTRL pESCs (orange) or 100 µM kainate–treated pESCs (KA, pink) and TSCs at the initial cytoneme-mediated contact (X c ) or 50 min after contact (X c+50 , Δ Distance = X c+50 – X c ). Bars are mean of n ≥ 58 cells pooled from ≥3 experiments. Error bars are SEM. Asterisks indicate statistical significance calculated by unpaired two-sided t test: **, P < 0.01.

Article Snippet: The antibodies used were anti-α-tubulin (YL1/2; rat; ab6160; Abcam), anti–β-catenin (mouse; #610154; BD Transduction), anti-LRP6 (EPR2423(2); rabbit; ab134146; Abcam), anti-NANOG (rabbit; RCAB002P-F; Reprocell), anti-OCT3/4 (mouse; #611202; BD Transduction), anti-EOMES (rabbit; ab183991; Abcam), anti-GriA3 (mouse; MAB5416; Sigma-Aldrich), anti-GriA4 (rabbit; AB1508; Sigma-Aldrich), anti-GriK1 (rabbit; AGC-008; Alomone Laboratories), anti-GriK3 (rabbit; AGC-040; Alomone Laboratories), anti–N-cadherin (mouse; #33-3900; Thermo Fisher Scientific), anti–E-cadherin (DECMA-1; rat; ab11512; Abcam), anti-GFP (chicken; GFP-1020; Aves), anti-mCherry (goat; #200-101-379; Rockland), and AF488, AF555, or AF647-conjugated secondary antibodies (Thermo Fisher Scientific).

Techniques: Activity Assay, Over Expression, Staining, RNA Expression, Live Cell Imaging

Primers used for RT-qPCR

Journal: The Journal of Cell Biology

Article Title: Pluripotency state regulates cytoneme selectivity and self-organization of embryonic stem cells

doi: 10.1083/jcb.202005095

Figure Lengend Snippet: Primers used for RT-qPCR

Article Snippet: The antibodies used were anti-α-tubulin (YL1/2; rat; ab6160; Abcam), anti–β-catenin (mouse; #610154; BD Transduction), anti-LRP6 (EPR2423(2); rabbit; ab134146; Abcam), anti-NANOG (rabbit; RCAB002P-F; Reprocell), anti-OCT3/4 (mouse; #611202; BD Transduction), anti-EOMES (rabbit; ab183991; Abcam), anti-GriA3 (mouse; MAB5416; Sigma-Aldrich), anti-GriA4 (rabbit; AB1508; Sigma-Aldrich), anti-GriK1 (rabbit; AGC-008; Alomone Laboratories), anti-GriK3 (rabbit; AGC-040; Alomone Laboratories), anti–N-cadherin (mouse; #33-3900; Thermo Fisher Scientific), anti–E-cadherin (DECMA-1; rat; ab11512; Abcam), anti-GFP (chicken; GFP-1020; Aves), anti-mCherry (goat; #200-101-379; Rockland), and AF488, AF555, or AF647-conjugated secondary antibodies (Thermo Fisher Scientific).

Techniques: Sequencing